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dc.contributor.authorHenning, Alyssa L.
dc.contributor.authorJiang, Michael X.
dc.contributor.authorYalçın, Hüseyin Çağatay
dc.contributor.authorButcher, Jonathan T.
dc.date.accessioned2015-01-15T07:33:17Z
dc.date.available2015-01-15T07:33:17Z
dc.date.issued2011-08
dc.identifier.citationHENNING, A.L., JIANG, M.X., YALÇIN, H.Ç., BUTCHER, J.T. (2011). Quantitative three-dimensional imaging of live avian embryonic morphogenesis via micro-computed tomography. Developmental Dynamics, 240 (8), 1949-1957. http://dx.doi.org/10.1002/dvdy.22694.en_US
dc.identifier.issn1058-8388
dc.identifier.other000292772400008 (WOS)
dc.identifier.other21761480 (PubMed)
dc.identifier.urihttp://dx.doi.org/10.1002/dvdy.22694
dc.identifier.urihttp://hdl.handle.net/11376/867
dc.description.abstractMany clinically relevant congenital malformations arise during mid to late embryonic stages. This period is challenging to image quantitatively in live embryos, necessitating the use of multiple specimens with increased experimental variability. Here we establish X-ray and blood-pool computed tomography (CT) contrast agent toxicity and teratogenesis thresholds for 3D Micro-CT imaging of live avian embryos. Day 4 chick embryos micro-injected with Visipaque(TM) (VP) developed for an additional 6 days without defect. X-ray radiation up to 798 mGy was nontoxic. Peak average contrast of 1,060 HU occurred within 1 hr of imaging at 50 mm resolution. VP-enhanced contrast persisted past 24 hr with delayed accumulation in the allantois. Regional volumes of VP-injected embryos were statistically identical to those of fixed embryos perfused with osmium tetroxide. We further quantified longitudinal volumetric morphogenesis of the allantois over 30 hr. These results demonstrate the safety and efficacy of contrast enhanced quantitative micro-CT imaging for live embryos.en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.isversionof10.1002/dvdy.22694en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectContrast Agenten_US
dc.subjectDevelopmenten_US
dc.subjectHearten_US
dc.subjectBrainen_US
dc.subjectToxicityen_US
dc.subjectRadiationen_US
dc.subjectTeratogenen_US
dc.subjectDefecten_US
dc.subjectDosageen_US
dc.subjectBiodistributionen_US
dc.subjectIodineen_US
dc.subjectAllantoisen_US
dc.subjectClearanceen_US
dc.titleQuantitative three-dimensional imaging of live avian embryonic morphogenesis via micro-computed tomographyen_US
dc.typearticleen_US
dc.relation.journalDevelopmental Dynamicsen_US
dc.contributor.departmentDoğuş Üniversitesi, Mühendislik Fakültesi, Makine Mühendisliği Bölümüen_US
dc.contributor.authorIDTR181531en_US
dc.identifier.volume240en_US
dc.identifier.issue8en_US
dc.identifier.startpage1949en_US
dc.identifier.endpage1957en_US


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